Detailed view for Tb927.7.220

Basic information

TDR Targets ID: 17457
Trypanosoma brucei, CDP-DAG synthase
Source Database / ID:  TriTrypDB  GeneDB

pI: 10.044 | Length (AA): 406 | MW (Da): 46350 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 6

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01148   Cytidylyltransferase family

Gene Ontology

Mouse over links to read term descriptions.
GO:0004605   phosphatidate cytidylyltransferase activity  
GO:0016772   transferase activity, transferring phosphorus-containing groups  
GO:0016020   membrane  
GO:0015450   P-P-bond-hydrolysis-driven protein transmembrane transporter activity  
GO:0015031   protein transport  

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_127441)

Species Accession Gene Product
Arabidopsis thaliana AT1G62430   phosphatidate cytidylyltransferase
Arabidopsis thaliana AT4G26770   phosphatidate cytidylyltransferase
Arabidopsis thaliana AT4G22340   cytidinediphosphate diacylglycerol synthase 2
Babesia bovis BBOV_II005070   cytidine diphosphate-diacylglycerol synthase, putative
Brugia malayi Bm1_36290   Putative phosphatidate cytidylyltransferase
Candida albicans CaO19.8866   CDP-diacylglycerol synthase
Candida albicans CaO19.1279   CDP-diacylglycerol synthase
Caenorhabditis elegans CELE_C33H5.18   Protein C33H5.18, isoform A
Cryptosporidium hominis Chro.70059   cytidine diphosphate-diacylglycerol synthase
Cryptosporidium parvum cgd7_450   putative cytidine diphosphate-diacylglycerol synthase; integral membrane protein with 7 or more transmembrane domains
Dictyostelium discoideum DDB_G0269742   CDP-diacylglycerol synthase
Drosophila melanogaster Dmel_CG7962   CDP diglyceride synthetase
Echinococcus granulosus EgrG_001128500   phosphatidate cytidylyltransferase
Echinococcus multilocularis EmuJ_001128500   phosphatidate cytidylyltransferase
Giardia lamblia GL50803_16906   Phosphatidate cytidylyltransferase
Homo sapiens ENSG00000101290   CDP-diacylglycerol synthase (phosphatidate cytidylyltransferase) 2
Homo sapiens ENSG00000163624   CDP-diacylglycerol synthase (phosphatidate cytidylyltransferase) 1
Leishmania braziliensis LbrM.26.1640   cdp-diacylglycerol synthetase-like protein
Leishmania donovani LdBPK_261600.1   CDP-DAG synthase, putative
Leishmania infantum LinJ.26.1600   cdp-diacylglycerol synthetase-like protein
Leishmania major LmjF.26.1620   cdp-diacylglycerol synthetase-like protein
Leishmania mexicana LmxM.26.1620   cdp-diacylglycerol synthetase-like protein
Loa Loa (eye worm) LOAG_03064   phosphatidate cytidylyltransferase
Mus musculus ENSMUSG00000029330   CDP-diacylglycerol synthase 1
Mus musculus ENSMUSG00000058793   CDP-diacylglycerol synthase (phosphatidate cytidylyltransferase) 2
Neospora caninum NCLIV_023660   Phosphatidate cytidylyltransferase (EC 2.7.7.41), related
Oryza sativa 4325310   Os01g0758400
Oryza sativa 4348326   Os10g0327300
Plasmodium berghei PBANKA_1032600   cytidine diphosphate-diacylglycerol synthase, putative
Plasmodium falciparum PF3D7_1409900   cytidine diphosphate-diacylglycerol synthase
Plasmodium knowlesi PKNH_1348400   cytidine diphosphate-diacylglycerol synthase
Plasmodium vivax PVX_085955   cytidine diphosphate-diacylglycerol synthase, putative
Plasmodium yoelii PY01816   phosphatidate cytidylyltransferase, putative
Saccharomyces cerevisiae YBR029C   phosphatidate cytidylyltransferase
Schistosoma japonicum Sjp_0001040   ko:K00981 phosphatidate cytidylyltransferase [EC2.7.7.41], putative
Schistosoma mansoni Smp_144030   phosphatidate cytidylyltransferase
Schmidtea mediterranea mk4.000129.13   Probable phosphatidate cytidylyltransferase
Trypanosoma brucei gambiense Tbg972.7.100   phosphatidate cytidylyltransferase
Trypanosoma brucei Tb927.7.220   CDP-DAG synthase
Trypanosoma cruzi TcCLB.511237.40   CDP-DAG synthase, putative
Toxoplasma gondii TGME49_281980   phosphatidate cytidylyltransferase
Theileria parva TP04_0048   phosphatidate cytidylyltransferase, putative
Trichomonas vaginalis TVAG_430160   phosphatidate cytidylyltransferase, putative
Trichomonas vaginalis TVAG_491120   phosphatidate cytidylyltransferase, putative

Essentiality

Tb927.7.220 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.7.220 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.7.220 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.7.220 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.7.220 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_C33H5.18 Caenorhabditis elegans larval arrest wormbase
CELE_C33H5.18 Caenorhabditis elegans larval lethal wormbase
CELE_C33H5.18 Caenorhabditis elegans sterile wormbase
YBR029C Saccharomyces cerevisiae inviable yeastgenome
PBANKA_1032600 Plasmodium berghei Essential plasmo
TGME49_281980 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.7.220 (Trypanosoma brucei), CDP-DAG synthase
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